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KMID : 0358619940280010091
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Korean Journal of Physiology
1994 Volume.28 No. 1 p.91 ~ p.97
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Production and Characterization of Specific Antibodies to Bombesin
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Kwon Hyeok-Yil
Lee Yun-Lyul
Park Hyoung-Jin
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Abstract
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In order to produce a specific bombesin antiserum far very sensitive radioimmunoassay, synthetic conjugated to bovine serum albumin was subcutaneously injected into guinea pigs. The conjugation was performed using either carbodiimide or gIutaraldehyde as a coupling agent. The antisera were characterized by analysis of Scatchard and Sips plots. The antiserum LBE 2G/2 raised by repeat injection of the immunogen conjugated with carbodiimide showed the titer of 1 : 188,000, very low cross-reactivity to bombesin-like peptides except bombesin, with high affinity constant and high heterogeneity index (0.91). The antiserum LBG 1G/2 produced by repeat injection of the immunogen conjugated with glutaraldehyde possessed the titer of 1 : 43,000, high cross-reactivity to some bombesin-like peptides, high affinity constant and high heterogeneity index (0.79). These results indicate that the antiserum LBE 2G/2 is specific only to bombesin and that the antiserum LBG IG/2 binds to some bombesin-like peptides such as alytesin, gastrin releasing peptide and neuromedin C. The antiserum LBE 2G/2 is sufficient for the very sensitive radioimmunoassay of bombesin.
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KEYWORD
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Bombesin antibody, Immunization technique, Radioimmunoassay
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